Goat anti Rabbit IgG (H + L) (HRP) (43C-CB1120)

Goat anti Rabbit IgG (H + L) secondary antibody (HRP)

Synonyms Goat anti Rabbit Immunoglobulin G (H + L) secondary antibody (HRP)
Specificity Rabbit IgG (H + L)
Cross Reactivity To be determined by end-user
Applications ELISA, IHC, WB
Immunogen Goat anti-rabbit IgG (H+L) (HRP) was raised in goat using rabbit IgG, whole molecule as the immunogen.

Images

Dot Blot analysis using Goat anti Rabbit IgG (H + L) (HRP) (43C-CB1120)

Shown here is a serial 1:1 dilution of Rabbit IgG protein (250ng starting total load) co-incubated with Goat anti Rabbit IgG (HRP) and Goat anti Rabbit (Dylight 649) 1:20,000. Blot was dried and imaged (A) on Biorad Versa Doc (30 sec, DyLight649), (B) LiCor Odyssey Reader (700 nm), (C ) Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec), (D) Incubated with TMB substrate TMBM for 5 minutes and scanned, and (E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager.

Specifications

Host Goat
Method of Purification Goat anti Rabbit IgG (H + L) (HRP) was purified by affinity chromatography.
Tag/Conjugate HRP
Form & Buffer Lyophilized from 0.02M K3PO4, pH 7.2 with 0.15M NaCl, 10 mg/ml BSA, and 0.01% gentamicin sulfate. DO NOT add NaN3. Immunoglobulin and protease free.

Usage & Assay Information

Usage Recommendations ELISA: 1:90,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains ? and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer.

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Images

  • Dot Blot analysis using Goat anti Rabbit IgG (H + L) (HRP) (43C-CB1120) | Shown here is a serial 1:1 dilution of Rabbit IgG protein (250ng starting total load) co-incubated with Goat anti Rabbit IgG (HRP) and Goat anti Rabbit (Dylight 649) 1:20,000. Blot was dried and imaged (A) on Biorad Versa Doc (30 sec, DyLight649), (B) LiCor Odyssey Reader (700 nm), (C ) Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec), (D) Incubated with TMB substrate TMBM for 5 minutes and scanned, and (E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager.
  • Western blot analysis using Goat anti Rabbit IgG (H + L) (HRP) (43C-CB1120) | Goat Anti Rabbit IgG (HRP)secondary antibody was used at 1:40,000 to detect a rabbit primary antibody by Western Blot. p27 antibody (1:1000 RT 30 minutes) showed detection of 0.1 ug of recombinant p27 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p27 fusion protein (arrow; expected MW: 73.3 kDa). Lane 3: MBP alone.

Availability: In stock

Price: £268.42
Size: 1 mg
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