Goat anti Rat IgG (H + L) (FITC) (43C-CB1216)

Goat anti Rat IgG (H + L) secondary antibody (FITC)

Synonyms Goat anti Rat Immunoglobulin G (H + L) secondary antibody (FITC)
Specificity Rat IgG (H + L)
Cross Reactivity To be determined by end-user
Applications FC, IF
Immunogen Goat anti-rat IgG (H+L) (FITC) was raised in goat using rat IgG whole molecule as the immunogen.

Images

Western blot analysis using Goat anti Rat IgG (H + L) (FITC) (43C-CB1216)

Western Blot of Fluorescein conjugated Rat IgG secondary antibody. Lane 1: Rat IgG. Lane 2: none. Load: 50 ng per lane. none. Fluorescein Goat secondary antibody at 1:1,000 for 60 min at RT. Block: Blocking buffer for 30 min at RT. Size: 25 & 55 kDa, 25 & 55 kDa for Rat IgG..

Specifications

Host Goat
Method of Purification Goat anti Rat IgG (H + L) (FITC) was purified by affinity chromatography.
Tag/Conjugate FITC
Form & Buffer Lyophilized. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2. 10 mg/ml Bovine Serum Albumin (BSA)-Immunoglobulin and Protease free. 0.01% (w/v) NaN3

Usage & Assay Information

Usage Recommendations FC: 1:2,000-1:10,000, IF: 1:500-1:2,500

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains ? and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer.

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Images

  • Western blot analysis using Goat anti Rat IgG (H + L) (FITC) (43C-CB1216) | Western Blot of Fluorescein conjugated Rat IgG secondary antibody. Lane 1: Rat IgG. Lane 2: none. Load: 50 ng per lane. none. Fluorescein Goat secondary antibody at 1:1,000 for 60 min at RT. Block: Blocking buffer for 30 min at RT. Size: 25 & 55 kDa, 25 & 55 kDa for Rat IgG..

Availability: In stock

Price: £148.23
Size: 2 mg
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