GroEL protein (30R-1016)
Purified recombinant E.coli GroEL protein
|Synonyms||mopA protein, 60 kDa chaperonin protein, groL protein, groEL protein, groEL protein protein, chaperonin groel, Protein Cpn60 protein|
Coomassie Blue stained SDS-PAGE of GroEL protein (30R-1016)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||MAAKDVKFGN DARVKMLRGV NVLADAVKVT LGPKGRNVVL DKSFGAPTIT KDGVSVAREI ELEDKFENMG AQMVKEVASK ANDAAGDGTT TATVLAQAII TEGLKAVAAG MNPMDLKRGI DKAVTAAVEE LKALSVPCSD SKAIAQVGTI SANSDETVGK LIAEAMDKVG KEGVITVEDG TGLQDELDVV EGMQFDRGYL SPYFINKPET GAVELESPFI LLADKKISNI REMLPVLEAV AKAGKPLLII AEDVEGEALA TLVVNTMRGI VKVAAVKAPG FGDRRKAMLQ DIATLTGGTV ISEEIGMELE KATLEDLGQA KRVVINKDTT TIIDGVGEEA AIQGRVAQIR QQIEEATSDY DREKLQERVA KLAGGVAVIK VGAATEVEMK EKKARVEDAL HATRAAVEEG VVAGGGVALI RVASKLADLR GQNEDQNVGI KVALRAMEAP LRQIVLNCGE EPSVVANTVK GGDGNYGYNA ATEEYGNMID MGILDPTKVT RSALQYAASV AGLMITTECM VTDLPKNDAA DLGAAGGMGG MGGMGGMM|
|Grade & Purity||> 95% pure|
|Molecular Weight||57.3 kDa (548aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 25mM Tris-HCl, pH7.5, containing 100mM NaCl, 5mM DTT, and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||GroEL protein is the major heat shock protein of E. coli and belongs to the chaperonin(HSP60) family. GroEL protein prevents misfolding of proteins and promotes the refolding and proper assembly of unfolded polypeptiedes generated under stress conditions. GroEL gene was amplified by PCR from E. coli and cloned into an expression vector. This protein was overexpressed in E. coli and was purified by using conventional chromatography techniques.|
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