GRPEL1 protein (His tag) (80R-1507)
Purified recombinant Human GRPEL1 protein
|Synonyms||HMGE, GRPEL1, GRPEL 1, GRPEL-1, GRPEL 1 protein, FLJ25609 protein, GRPEL-1 protein, grpE protein homolog 1 mitochondrial protein|
Coomassie Blue stained SDS-PAGE of GRPEL1 protein (His tag) (80R-1507)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||28-217 amino acids: MGSSHHHHHH SSGLVPRGSH MCTATKQKNS GQNLEEDMGQ SEQKADPPAT EKTLLEEKVK LEEQLKETVE KYKRALADTE NLRQRSQKLV EEAKLYGIQA FCKDLLEVAD VLEKATQCVP KEEIKDDNPH LKNLYEGLVM TEVQIQKVFT KHGLLKLNPV GAKFDPYEHE ALFHTPVEGK EPGTVALVSK VGYKLHGRTL RPALVGVVKE A|
|Grade & Purity||> 90% pure|
|Molecular Weight||23.6 kDa (211aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 0.2M NaCl, 1mM DTT and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||GRPEL1 is an essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. This protein seems to control the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins. Recombinant human GRPEL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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