GSTP1 protein (His tag) (80R-1163)

Purified recombinant Human GSTP1 protein

Synonyms GSTP1, FAEES3 protein, Glutathione S-transferase P protein, Glutathione S Transferase Pi protein, GSTP1., GSTP-1, PI protein, GSTP-1 protein, DFN7 protein, GST3 protein, GSTP 1 protein, GSTP 1, Glutathione S-transferase P Fatty Acid Ethyl Ester Synthase III protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Prepare a 1ml reaction mix in a suitable container: The final concentrations are 97 mM potassium phosphate, 0.97 mM EDTA, 2.5 mM glutathione, reduced, 1.0 mM 1-chloro-2,4-dinitrobenzene (CDNB), 3.2% (v/v) ethanol. 2. Equilibrate to 25 DEG C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 50ul of recombinant GSTP1 protein with various concentrations (1ug, 2ug, 5ug) in 950ul reaction buffer. 4. Mix by inversion and record the increase at A340nm for 5 minutes.

Images

SDS-PAGE analysis of GSTP1 protein (His tag) (80R-1163)

SDS-PAGE gel analysis of GSTP1 protein (His tag) where the figure is annotated with amount of protein run on the gel.

Specifications

Residues 1-210 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMPP YTVVYFPVRG RCAALRMLLA DQGQSWKEEV VTVETWQEGS LKASCLYGQL PKFQDGDLTL YQSNTILRHL GRTLGLYGKD QQEAALVDMV NDGVEDLRCK YISLIYTNYE AGKDDYVKAL PGQLKPFETL LSQNQGGKTF IVGDQISFAD YNLLDLLLIH EVLAPGCLDA FPLLSAYVGR LSARPKLKAF LASPEYVNLP INGNGKQ
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 27.6 kDa (247 aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 7.0, containing 30% glycerol, 1mM EDTA, and 0.1mM PMSF.
Concentration 0.5 mg/ml

Usage & Assay Information

Bioactivity Specific activity is < 6.5 units/mg, and is defined as the amount of enzyme that conjugate 1.0 uM of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25 DEG C.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels < 1.0 EU per ug of protein (determined by LAL method)

General Information

Biological Significance GSTP1 is a glutathione S-transferase that belongs to the pi class. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide variety of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. Recombinant GSTP1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • SDS-PAGE analysis of GSTP1 protein (His tag) (80R-1163) | SDS-PAGE gel analysis of GSTP1 protein (His tag) where the figure is annotated with amount of protein run on the gel.

Availability: In stock

Price: £316.49
Size: 100 ug
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