hchA protein (His tag) (80R-1780)
Purified recombinant E.coli hchA protein
|Synonyms||Hsp31 molecular chaperone protein, yedU protein, yzzC protein, ECK1963 protein, JW1950 protein|
Coomassie Blue stained SDS-PAGE of hchA protein (His tag) (80R-1780)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-283 amino acids: MGSSHHHHHH SSGLVPRGSH MTVQTSKNPQ VDIAEDNAFF PSEYSLSQYT SPVSDLDGVD YPKPYRGKHK ILVIAADERY LPTDNGKLFS TGNHPIETLL PLYHLHAAGF EFEVATISGL MTKFEYWAMP HKDEKVMPFF EQHKSLFRNP KKLADVVASL NADSEYAAIF VPGGHGALIG LPESQDVAAA LQWAIKNDRF VISLCHGPAA FLALRHGDNP LNGYSICAFP DAADKQTPEI GYMPGHLTWY FGEELKKMGM NIINDDITGR VHKDRKLLTG DSPFAANALG KLAAQEMLAA YAG|
|Grade & Purity||> 95% pure|
|Molecular Weight||33.3 kDa(303aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 0.1M NaCl and 1mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||HchA, also known as, Escherichia coli Hsp31, is a homodimeric member of the ThiI/DJ-1/PfpI superfamily that combines molecular chaperone and aminopeptidase activities. It uses temperature-induced exposure of structured hydrophobic domains to capture and stabilize early unfolding protein intermediates under severe thermal stress. Recombinant E. coli hchA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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