Heme oxygenase 1 protein (His tag) (80R-1099)
Purified recombinant Human Heme oxygenase 1 protein
|Synonyms||HMOX 1 protein, Heme oxygenase (decycling) 1 protein, Heat shock protein 32 protein, HO1., Hemox protein, bK286B10 protein, HSP32 protein, HO protein, HO 1 protein, D8Wsu38e protein, HMOX1 protein, Heme oxygenase 1 protein, HO-1 protein, Hmox protein|
Coomassie Blue stained SDS-PAGE of Heme oxygenase 1 protein (His tag) (80R-1099)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-266 amino acids: MERPQPHSMP QDLSEALKEA TKEVHTQAEN AEFMRNFQKG QVTRDGFKLV MASLYHIYVA LEEEIERNKE SPVFAPVYFP EELHRKAALE QDLAFWYGPR WQEVIPYTPA MQRYVKRLHE VGRTEPELLV AHAYTRYLGD LSGGQVLKKI AQKALDLPSS GEGLAFFTFP NIASATKFKQ LYRSRMNSLE MTPAVRQRVI EEAKTAFLLN IQLFEELQEL LTHDTKDQSP SRAPGLRQRA SNKVQDSAPV ETPRGKPPLN TRSQAPLEHH HHHH|
|Grade & Purity||> 95% pure|
|Molecular Weight||31.4 kDa (274 aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 50mM NaCl, 0.1mM PMSF, and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Endotoxin Levels||< 1.0 EU per ug of protein (determined by LAL method)|
|Biological Significance||Heme oxygenase 1 belongs to the heme oxygenase family and is an essential enzyme in heme catabolism. It cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Also this protein is known to play an important role in the regulation of cardiovascular function and its adaptive response to a variety of stressors. Recombinant human Heme oxygenase 1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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