Hepatitis C Virus NS3 protein (His tag) (80R-1286)

Purified recombinant Hepatitis C Virus NS3 protein (His tag)

Synonyms NS 3 protein, NS3P protein, Hepatitis C protein, P70 protein, Serine protease/NTPase/helicase., NS3 protease/helicase HCV NS3 transactivated protein protein
Species Viral
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of HCV NS3 protein (His tag) (80R-1286)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1225-1456 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSVAHL HAPTGSGKST KVPAAYAAQG YKVLVLNPSV AATLGFGAYM SKAHGVDPNI RTGVRTITTG SPITYSTYGK FLADGGCSGG AYDIIICDEC HSTDATSILG IGTVLDQAET AGARLVVLAT ATPPGSVTVS HPNIEEVALS TTGEIPFYGK AIPLEVIKGG RHLIFCHSKK KCDELAAKLV ALGINAVAYY RGLDVSVIPT SGDVVVVSTD ALMTGFTGDF DSVIDCNT
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 27.9 kDa (268aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, and 1mM DTT.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. Nonstructural (NS) proteins of HCV play major roles in viral replication and the pathogenesis of liver diseases. The NS3 protein of hepatitis C virus (HCV) contains protease and RNA helicase activities, both of which are likely to be essential for HCV propagation. Recombinant HCV NS3 protein was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of HCV NS3 protein (His tag) (80R-1286) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £272.43
Size: 100 ug
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