Hexokinase antibody (10R-1056)

Mouse monoclonal Hexokinase antibody

Synonyms Monoclonal Hexokinase antibody, Anti-Hexokinase antibody, Brain form hexokinase antibody, Glycolytic enzyme antibody, Hexokinase PI antibody, Hexokinase-A antibody, Hexokinase-1 antibody, Hexokinase type I antibody, HK I antibody, HK1 tb antibody, tumor isozyme antibody, Hexokinase type I antibody, EC 2.7.1.1 antibody, mHk1-s. antibody, Hexokinase 1 antibody, dea antibody, HKI antibody, BB404130 antibody, Hk1-s antibody, HXK1 antibody, HK1-tc antibody, Hexokinase antibody, HK1 antibody, HK1-ta antibody, HEXOKIN antibody
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, WB
Immunogen Hexokinase antibody was raised in mouse using recombinant human Hexokinase1 (1-917aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of Hexokinase antibody (10R-1056)

(A): Recombinant protein (20ng or 100ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human Hexokinase (1:1000). (B): Cell lysates (20 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human Hexokinase (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone 4D7
Isotype IgG2a kappa
Source Anti-human Hexokinase1 antibody, clone 4D7, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Hexokinase1 protein.
Method of Purification Hexokinase antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations To be determined by end-user.

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-type 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this hexokinase1 have been associated with hemolytic anemia due to hexokinase deficiency.

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Images

  • Western Blot analysis of Hexokinase antibody (10R-1056) | (A): Recombinant protein (20ng or 100ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human Hexokinase (1:1000). (B): Cell lysates (20 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human Hexokinase (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: £212.33
Size: 100 ul
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