HIF1A protein (His tag) (80R-1409)

Purified recombinant Human HIF1A protein

Synonyms HIFA-1 protein, HIFA 1 protein, BHLHE78 protein, HIFA-1, Hypoxia-inducible factor 1-alpha, PASD8 protein, HIFA 1, HIF1A, MOP1 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of HIF1A protein (His tag) (80R-1409)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 576-785 amino acids: MGSSHHHHHH SSGLVPRGSH MSFDQLSPLE SSSASPESAS PQSTVTVFQQ TQIQEPTANA TTTTATTDEL KTVTKDRMED IKILIASPSP THIHKETTSA TSSPYRDTQS RTASPNRAGK GVIEQTEKSH PRSPNVLSVA LSQRTTVPEE ELNPKILALQ NAQRKRKMEH DGSLFQAVGI GTLLQQPDDH AATTSLSWKR VKGCKSSEQN GMEQKTIILI PSDLACRLLG Q
Expression System E.coli
Grade & Purity > 85% pure
Molecular Weight 25.1 kDa (231aa) confirmed by MALDI-TOF (Real molecular weight on SDS-PAGE will be shift up)
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 0.2M NaCl, 1mM DTT and 10% glycerol.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance HIF1A, identified as a transcription factor, has been found to play an essential role in oxygen homeostasis. This protein is a heterodimer composed of HIF-1beta subunit and one of three subunits (HIF-1A, HIF-2A or HIF-3A). The activation of HIF1A is closely associated with a variety of tumors and oncogenic pathways. HIF1A consists of DNA binding domain (DBD domain), Dimerization domain and C-terminla regulatiory domains, including two transactivation domains (TAD), an oxygen-dependent degradation (ODD) domain, and inhibitory domains. Recombinant human HIF1A (576-785) protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

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Images

  • Coomassie Blue stained SDS-PAGE of HIF1A protein (His tag) (80R-1409) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £204.32
Size: 50 ug
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