His Tag antibody (20R-HC001)

Chicken polyclonal His Tag antibody

Synonyms Polyclonal His Tag antibody, Anti-His Tag antibody, HHHHHH antibody, Polyhistidine Tag antibody, 6XHis antibody, HIS6 Tag antibody, 6-His antibody
Cross Reactivity To be determined by end-user
Applications ELISA, ICC, WB
Immunogen His tag antibody was raised in chicken using 6-HIS (HHHHHH) conjugated to KLH as the immunogen.

Specifications

Host Chicken
Isotype IgG
Method of Purification His Tag antibody was purified by affinity chromatography.
Form & Buffer Supplied as a liquid in PBS, pH 7.2 with 0.1% NaN3.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: coating:1:100-1:500, primary:1:1,000-1:30,000, ICC: 1:100-1:400, WB: colorimetric: 1:1,000-1:10,000, chemiluminescent: 1:1,000-1:30,000

Storage & Safety

Storage Store at 4 deg C.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

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Availability: In stock

Price: £300.47
Size: 100 ug
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