HMGB1 antibody (10R-1114)

Mouse monoclonal HMGB1 antibody

Synonyms Monoclonal HMGB1 antibody, Anti-HMGB1 antibody, Amphoterin antibody, HMGB -1 antibody, HMGB 1, HMGB1 antibody, HMGB -1, High-mobility group box1 antibody, High-mobility group box1 antibody, HMGB 1 antibody, HMGB1, high-mobility group (nonhistone chromosomal) protein 1 antibody, DKFZp686A04236 antibody, Sulfoglucuronyl carbohydrate binding protein antibody, high mobility group protein 1 antibody, HMG3 antibody, SBP-1 antibody, HMG1 antibody
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, IHC, WB
Immunogen HMGB1 antibody was raised in mouse using recombinant human HMGB1 (1-215aa) purified from Trichoplusia ni insect cells as the immunogen.

Images

Western Blot analysis of HMGB1 antibody (10R-1114)

Cell lysates of Jurkat and HeLa (30 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human HMGB1 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone J2E1
Isotype IgG2b kappa
Source Anti-human HMGB1 antibody, clone J2E1, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HMGB1 protein.
Method of Purification HMGB1 antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IHC: 1:100-1:300, WB: 1:500-1:2,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance High mobility group box1 protein (HMGB1) is a very abundant chromatin-binding protein residing in the eukaryotic cell nucleus and acting in the assembly of nucleoprotein complexes. Inside the cell, HMGB1 binds to DNA and has a role in transcriptional regulation. Outside the cell, HMGB1 acts as a cytokine and has activities that resemble those of tumor necrosis factor.

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Images

  • Western Blot analysis of HMGB1 antibody (10R-1114) | Cell lysates of Jurkat and HeLa (30 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human HMGB1 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: £236.37
Size: 100 ul
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