HPRT1 protein (His tag) (80R-1348)

Purified recombinant Human HPRT1 protein

Synonyms Hypoxanthine-guanine phosphoribosyltransferase HPRT 1 protein, HGPRTase protein, Hypoxanthine phosphoribosyltransferase 1 (Lesch Nyhan syndrome) protein, Hypoxanthine phosphoribosyltransferase 1., HPRT-1 protein, HPRT1 protein, HPRT1, HPRT 1, Hypoxanthine guanine phosphoribosyltransferase protein, HPRT protein, HPRT-1, HPRT 1 protein, HGPRT protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of HPRT1 protein (His tag) (80R-1348)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-218 amino acids: MGSSHHHHHH SSGLVPRGSH MATRSPGVVI SDDEPGYDLD LFCIPNHYAE DLERVFIPHG LIMDRTERLA RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV RQYNPKMVKV ASLLVKRTPR SVGYKPDFVG FEIPDKFVVG YALDYNEYFR DLNHVCVISE TGKAKYKA
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 26.7 kDa (238aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol.
Concentration 0.5 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Hypoxanthine-guanine phosphoribosyltransferase, also known as HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. The enzyme primarily functions to salvage purines from degraded DNA to renewed purine synthesis. In this role, it acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP. Recombinant human HPRT1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of HPRT1 protein (His tag) (80R-1348) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £236.37
Size: 100 ug
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