HPRT1 protein (His tag) (80R-1348)
Purified recombinant Human HPRT1 protein
|Synonyms||Hypoxanthine-guanine phosphoribosyltransferase HPRT 1 protein, HGPRTase protein, Hypoxanthine phosphoribosyltransferase 1 (Lesch Nyhan syndrome) protein, Hypoxanthine phosphoribosyltransferase 1., HPRT-1 protein, HPRT1 protein, HPRT1, HPRT 1, Hypoxanthine guanine phosphoribosyltransferase protein, HPRT protein, HPRT-1, HPRT 1 protein, HGPRT protein|
Coomassie Blue stained SDS-PAGE of HPRT1 protein (His tag) (80R-1348)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-218 amino acids: MGSSHHHHHH SSGLVPRGSH MATRSPGVVI SDDEPGYDLD LFCIPNHYAE DLERVFIPHG LIMDRTERLA RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV RQYNPKMVKV ASLLVKRTPR SVGYKPDFVG FEIPDKFVVG YALDYNEYFR DLNHVCVISE TGKAKYKA|
|Grade & Purity||> 95% pure|
|Molecular Weight||26.7 kDa (238aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Hypoxanthine-guanine phosphoribosyltransferase, also known as HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. The enzyme primarily functions to salvage purines from degraded DNA to renewed purine synthesis. In this role, it acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP. Recombinant human HPRT1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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