Human IgG ELISA Kit (55R-M1551clb)

ELISA kit for detection of Human IgG in the research laboratory

Synonyms Human Immunoglobulin G ELISA Kit
Applications ELISA

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user

Storage & Safety

Storage Store at 4 deg C.

General Information

Biological Significance This ELISA kit is a sandwich-type enzyme immunoassay. The kit contains microwell strips coated with highly avid monoclonal antibodies, each specific for one of the human IgG subclasses. Test samples, calibrator- and control sera are incubated in the respective wells. The IgG subclass to be determined will bind to the solid phase and non-bound IgG is removed by washing. Next, peroxidase-conjugated anti-human IgG antiserum is added to each well and non-bound conjugate is removed by washing. After incubation with substrate solution (ABTS) and H2O2, the reaction is stopped with an acid buffer. The green colored reaction product is measured by absorbance and the concentration of IgG subclass in the test sample calculated relative to the values of a reference curve. IgG subclass control serum is assayed to check the validity of the calibration curves and the accuracy of the IgG subclass determinations.

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Human IgG ELISA Kit Protocol

Added: Jun 22, 2015

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Availability: In stock

Price: £460.72
Size: 1 kit
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