IGF1 protein (30R-1058)

Purified recombinant Human IGF1 protein

Synonyms MGF protein, IGF 1 protein, IGF-IA protein, Insulin like growth factor 1 protein, Insulin like growth factor 1 (somatomedin C) protein, Insulin like growth factor IB protein, IGF1 protein, IGFI protein, IGF IB protein, IGF1, IGF-1, IGF 1, IBP1 protein, IGF-1 protein, Insulin-like growth factor-1 protein, IGF1 protein, Mechano growth factor protein, IGF-1 protein, , Somatomedin C protein, IGFIA protein, Insulin like growth factor IA protein, Insulin-like growth factor-1 IGF IA protein, IBP1 protein, Somatomedin-C protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information Cell line: MCF7 (human) Maintenance Condition: RPMI 1640 containing 10% FBS Assay Medium: serum free RPMI 1640 Cell Density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) Serum Free Starvation: 24hr with RPMI1640 Incubation Time: 40 hr (after sample treatment) Concentration Range: 0.39ng/ml - 25ng/ml Detection Method: BrdU assay

Images

Coomassie Blue stained SDS-PAGE of IGF1 protein (30R-1058)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-115 amino acids: MGPETLCGAE LVDALQFVCG DRGFYFNKPT GYGSSSRRAP QTGIVDECCF RSCDLRRLEM YCAPLKPAKS A
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 7.7 kDa (71aa), confirmed by MALDI-TOF.
Form & Buffer Supplied as a liquid in PBS, pH 7.4.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity The ED50 for this effect is < 3.6 ng/ml. Measured in a cell proliferation assay using MCF7 cell.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The Insulin-like growth factor-1(IGF-1) is a mitogenic polypeptide growth factors that stimulates the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGF-1 is predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGF-1 belongs to the insulin gene family, which also contains insulin and relaxin. The IGF-1s are similar in structure and function to insulin, but have a much higher growth-promoting activity than insulin. This recombinant human IGF-I is a globular protein containing 70 amino acids, and 3 intra-molecular disulfide bonds. Recombinant IGF-1 was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

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Images

  • Coomassie Blue stained SDS-PAGE of IGF1 protein (30R-1058) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £144.23
Size: 100 ug
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