IkBa antibody (20-IR25)

Rabbit polyclonal IkBa antibody

Synonyms Polyclonal IkBa antibody, Anti-IkBa antibody, IkappaBalpha antibody, NFKBI antibody, NFKBIA antibody, IKBalpha antibody, NF kappa B inhibitor alpha antibody, MAD3 antibody, IKBA antibody
Specificity Human IkBa
Cross Reactivity To be determined by end-user
Applications ELISA, IHC, WB
Immunogen IkBa antibody was raised in rabbit using IkBa peptide corresponding to a region near the C-terminus of the human protein conjugated to KLH as the immunogen.

Images

Western blot analysis using IkBa antibody (20-IR25)

Myeloperoxidase [Human Leukocytes] antibody detects multiple MPO subunits and chain combinations by western blot. Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kDa and 15 kDa polypeptides and chain combinations forming 68 kDa and 106 kDa proteins. The staining of the 68 kDa band is so intense that is over saturates the signal detection. A 4-20% gradient gel was used to separate the protein by SDS-PAGE.

Specifications

Host Rabbit
Method of Purification IkBa antibody was purified by delipidation and defibrination.
Form & Buffer 0.02M K3PO4,pH 7.2, with 0.15M NaCl, and 0.01%NaN3.
Concentration 80.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000

Storage & Safety

Storage Store at -20 deg C. Aliquot and freeze at -20 deg C or below for long term storage. Avoid repeated freeze/thaw cycles.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance I?B alpha (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha) is one member of a family of cellular proteins that function to inhibit the NF-?B transcription factor. I?B alpha inhibits NF-?B by masking the nuclear localization signals (NLS) of NF-?B proteins and keeping them sequestered in an inactive state in the cytoplasm.

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Images

  • Western blot analysis using IkBa antibody (20-IR25) | Myeloperoxidase [Human Leukocytes] antibody detects multiple MPO subunits and chain combinations by western blot. Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kDa and 15 kDa polypeptides and chain combinations forming 68 kDa and 106 kDa proteins. The staining of the 68 kDa band is so intense that is over saturates the signal detection. A 4-20% gradient gel was used to separate the protein by SDS-PAGE.

Availability: In stock

Price: £460.72
Size: 100 ul
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