KARS protein (His tag) (80R-1927)

Purified recombinant KARS protein

Synonyms Lysyl-tRNA synthetase protein, KARS2 protein, CMTRIB protein, KRS protein, KIAA0070 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information KARS protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Images

Coomassie Blue stained SDS-PAGE of KARS protein (His tag) (80R-1927)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 63-597 amino acids: MGSSHHHHHH SSGLVPRGSH MGSGVGPEEE SVDPNQYYKI RSQAIHQLKV NGEDPYPHKF HVDISLTDFI QKYSHLQPGD HLTDITLKVA GRIHAKRASG GKLIFYDLRG EGVKLQVMAN SRNYKSEEEF IHINNKLRRG DIIGVQGNPG KTKKGELSII PYEITLLSPC LHMLPHLHFG LKDKETRYRQ RYLDLILNDF VRQKFIIRSK IITYIRSFLD ELGFLEIETP MMNIIPGGAV AKPFITYHNE LDMNLYMRIA PELYHKMLVV GGIDRVYEIG RQFRNEGIDL THNPEFTTCE FYMAYADYHD LMEITEKMVS GMVKHITGSY KVTYHPDGPE GQAYDVDFTP PFRRINMVEE LEKALGMKLP ETNLFETEET RKILDDICVA KAVECPPPRT TARLLDKLVG EFLEVTCINP TFICDHPQIM SPLAKWHRSK EGLTERFELF VMKKEICNAY TELNDPMRQR QLFEEQAKAK AAGDDEAMFI DENFCTALEY GLPPTAGWGM GIDRVAMFLT DSNNIKEVLL FPAMKPEDKK ENVATTDTLE STTVGTSV
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 63.7 kDa
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, with 20% glycerol, 0.1M NaCl and 1mM DTT.
Concentration 1.0 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C or -70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance KARS, also known as Lysyl-tRNA synthetase, belongs to the class-II aminoacyl-tRNA synthetase family. It exists as both mitochondrial and cytoplasmic isoforms produced by alternative splicing, and thought to play a role in autoimmune diseases, such as polymyositis or dermatomyositis. This protein functions to catalyze the aminoacylation of tRNAs by their corresponding amino acids, thus linking amino acids with tRNA-contained nucleotide triplets. Recombinant human KARS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of KARS protein (His tag) (80R-1927) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £204.32
Size: 100 ug
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