KARS protein (His tag) (80R-1927)
Purified recombinant KARS protein
|Synonyms||Lysyl-tRNA synthetase protein, KARS2 protein, CMTRIB protein, KRS protein, KIAA0070 protein|
|Assay Information||KARS protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.|
Coomassie Blue stained SDS-PAGE of KARS protein (His tag) (80R-1927)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||63-597 amino acids: MGSSHHHHHH SSGLVPRGSH MGSGVGPEEE SVDPNQYYKI RSQAIHQLKV NGEDPYPHKF HVDISLTDFI QKYSHLQPGD HLTDITLKVA GRIHAKRASG GKLIFYDLRG EGVKLQVMAN SRNYKSEEEF IHINNKLRRG DIIGVQGNPG KTKKGELSII PYEITLLSPC LHMLPHLHFG LKDKETRYRQ RYLDLILNDF VRQKFIIRSK IITYIRSFLD ELGFLEIETP MMNIIPGGAV AKPFITYHNE LDMNLYMRIA PELYHKMLVV GGIDRVYEIG RQFRNEGIDL THNPEFTTCE FYMAYADYHD LMEITEKMVS GMVKHITGSY KVTYHPDGPE GQAYDVDFTP PFRRINMVEE LEKALGMKLP ETNLFETEET RKILDDICVA KAVECPPPRT TARLLDKLVG EFLEVTCINP TFICDHPQIM SPLAKWHRSK EGLTERFELF VMKKEICNAY TELNDPMRQR QLFEEQAKAK AAGDDEAMFI DENFCTALEY GLPPTAGWGM GIDRVAMFLT DSNNIKEVLL FPAMKPEDKK ENVATTDTLE STTVGTSV|
|Grade & Purity||> 95% pure|
|Molecular Weight||63.7 kDa|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, with 20% glycerol, 0.1M NaCl and 1mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Aliquot and store at -20 deg C or -70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||KARS, also known as Lysyl-tRNA synthetase, belongs to the class-II aminoacyl-tRNA synthetase family. It exists as both mitochondrial and cytoplasmic isoforms produced by alternative splicing, and thought to play a role in autoimmune diseases, such as polymyositis or dermatomyositis. This protein functions to catalyze the aminoacylation of tRNAs by their corresponding amino acids, thus linking amino acids with tRNA-contained nucleotide triplets. Recombinant human KARS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.|
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