KCTD15 antibody (10R-1175)

Mouse monoclonal KCTD15 antibody

Synonyms Monoclonal KCTD15 antibody, Anti-KCTD15 antibody, KCTD 15 antibody, KCTD15, KCTD-15, potassium channel tetramerisation domain containing 15 antibody, KCTD-15 antibody, MGC25497 antibody, BTB/POZ domain-containing protein KCTD15 antibody, MGC2628 antibody, KCTD 15
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, IF, WB
Immunogen KCTD15 antibody was raised in mouse using recombinant human KCTD15 (1-234) purified from E.coli as the immunogen.

Images

Immunofluorescent staining using KCTD15 antibody (10R-1175)

Immunofluorescenitrocellulosee of human HeLa cells stained with monoclonal anti-human KCDT15 antibody (1:500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).

Specifications

Host Mouse
Clone AT4C3
Isotype IgG3 gamma
Source Anti-human KCTD15 antibody, clone AT4C3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human KCTD15 protein.
Method of Purification KCTD15 antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF: 1:250-1:500, WB: 1:250-1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance KCTD15 (Potassium channel tetramerisation domain containing 15, also known as BTB/POZ domain-containing protein KCTD15) is protein that in humans is encoded by the KCTD15 gene. KCTD15 is expressed at high level in brain and hypothalamus. The potassium channel KCTD15 was identified as a genetic loci associated with higher than normal body mass index (BMI) in humans along with genes such as GNPDA2, MTCH2, FTO, and TMEM18. Single nucleotide polymorphisms (SNPs) in non-diabetic and diabetic patients showed that FTO was most strongly associated with obesity while MTCH2 and GNPDA2 were still significantly associated with higher than normal BMI levels.

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Images

  • Immunofluorescent staining using KCTD15 antibody (10R-1175) | Immunofluorescenitrocellulosee of human HeLa cells stained with monoclonal anti-human KCDT15 antibody (1:500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).
  • Western Blot analysis of KCTD15 antibody (10R-1175) | Cell lysates of HeLa (35 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human KCTD15 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: £236.37
Size: 100 ul
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