KI67 antibody (20R-KR001)
Rabbit polyclonal KI67 antibody
|Synonyms||Polyclonal KI67 antibody, Anti-KI67 antibody, KI-67, KI67, KI-67 antibody, KI 67 antibody, KI 67|
|Immunogen||KI67 antibody was raised in rabbit using a synthetic peptide derived from human Ki-67 Protein as the immunogen.|
Immunohistochemical staining using KI67 antibody (20R-KR001)
Formalin-fixed, paraffin-embedded human tonsil stained with Ki67 antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of proliferating cells.
|Method of Purification||KI67 antibody was purified by Protein A chromatography.|
|Form & Buffer||Supplied as a liquid with 0.2% BSA and 15mM NaN3.|
Usage & Assay Information
|Usage Recommendations||IHC-F: 10-20 ug/ml, IHC-P: 10-20 ug/ml|
Storage & Safety
|Storage||Store at 4 deg C.|
|Biohazard Information||This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling.|
|Biological Significance||Ki-67 is a nuclear protein, which is expressed in proliferating cells. Ki-67 is preferentially expressed during late G1-, S-, M-, and G2-phases of the cell cycle, while cells in the G0 (quiescent) phase are negative for this protein. During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes.|
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