LDH antibody (20C-CR6062GP)

Goat polyclonal LDH antibody

Synonyms Polyclonal LDH antibody, Anti-LDH antibody, L-lactate dehydrogenase A chain antibody, LDH-A antibody, LDH-M antibody, LDH muscle subunit antibody
Specificity Rabbit LDH
Cross Reactivity To be determined by end-user
Applications ELISA, IP, WB
Immunogen LDH antibody was raised in goat using full length lactate dehydrogenase protein isolated from rabbit muscle as the immunogen.

Specifications

Host Goat
Method of Purification LDH antibody was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Form & Buffer IgG fraction supplied as a liquid in PBS, pH 7.4, with 0.01% NaN3

Usage & Assay Information

Usage Recommendations ELISA: 1:2,000-1:10,000, IP: 1:100, WB: 1:1,000-1:5,000

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Lactate dehydrogenase is also known as L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit and LDH-M. Two isozymes of LDH occur in mammals, LDH-M and LDH-H which come together to form a homotetramer of 36 kDa subunits. Every LDH molecule consists of four subunits, where each subunit is either H or M (based on their electrophoretic properties.) There are, therefore, five LDH isotypes: LDH-1 (4H)-in the heart, LDH-2 (3H1M)-in the reticuloendothelial system, LDH-3 (2H2M)-in the lungs, LDH-4 (1H3M)-in the kidneys and LDH-5 (4M)-in the liver and striated muscle. Usually LDH-2 is the predominant form in the serum. An LDH-1 level higher than the LDH-2 level (a "flipped pattern") suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH-1, into the bloodstream). In general, LDH

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Availability: In stock

Price: £84.13
Size: 1 ml
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