LDHA antibody (10R-1177)

Mouse monoclonal LDHA antibody

Synonyms Monoclonal LDHA antibody, Anti-LDHA antibody, Lactate dehydrogenase M antibody, Lactate dehydrogenase A4 antibody, Proliferation-inducing gene 19 antibody, l7R2 antibody, A chain antibody, LDHM antibody, GSD11 antibody, LDH1 antibody, LDH muscle subunit antibody, Proliferation-inducing gene 19 antibody, OTTMUSP00000017774 antibody, A chain antibody, Lactate dehydrogenase A antibody, L-lactate dehydrogenase A chain Cell proliferation-inducing gene 19 protein antibody, Renal carcinoma antigen NY-REN-59. antibody, M LDH antibody, Lactate dehydrogenase M antibody, L lactate dehydrogenase A chain antibody, L lactate dehydrogenase A chain antibody, LDHA antibody, GSD11 antibody, Anti LDHA antibody , LDH muscle subunit antibody, OTTMUSP00000017774 antibody, Lactate dehydrogenase 1 antibody, LDHM antibody, Renal carcinoma antigen NY-REN-59. antibody, PIG19 antibody, l7R2 antibody, LDH1 antibody, M LDH antibody, Lactate dehydrogenase A antibody, LDH muscle subunit antibody, L-lactate dehydrogenase A chain Cell proliferation-inducing gene 19 protein antibody, Lactate dehydrogenase A4 antibody, Lactate dehydrogenase 1 antibody, LDH muscle subunit antibody, PIG19 antibody
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, IF, WB
Immunogen LDHA antibody was raised in mouse using recombinant human LDHA (1-332aa) purified from E.coli as the immunogen.

Images

Immunofluorescent staining using LDHA antibody (10R-1177)

Immunofluorescenitrocellulosee of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human LDHA antibody (1:500) with Texas Red (Red).

Specifications

Host Mouse
Clone AT1A4
Isotype IgG2b kappa
Source Anti-human LDHA antibody, clone AT1A4, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human LDHA protein.
Method of Purification LDHA antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF: 1:500-1:8,000, WB: 1:500-1:8,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Lactate through lactate dehydrogenase A (LDHA), which is encoded by a target gene of c-Myc and hypoxia-inducible factor (HIF-1). LDHA expression is repressed by SugR in the absence of sugar. Reduction of LDHA causes bioenergetic and oxidative stress leading to cell death. LDHA (LDH-5, M-LDH, or A4), which is the predominant form in skeletal muscle, kinetically favors the conversion of pyruvate to lactate. Therefore, LDH-A is an attractive target for cancer therapy since its expression is largely relegated to skeletal muscle.

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Images

  • Immunofluorescent staining using LDHA antibody (10R-1177) | Immunofluorescenitrocellulosee of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human LDHA antibody (1:500) with Texas Red (Red).
  • Western Blot analysis of LDHA antibody (10R-1177) | Cell lysates of HeLa (35 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human LDHA (1:8000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: £212.33
Size: 100 ul
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