MCSF protein (His tag) (80R-1225)

Purified recombinant Human MCSF protein

Synonyms CSF 1 protein, Macrophage colony stimulating factor Colony stimulating factor 1 protein, MCSF protein, MGC31930., Lanimostim protein, M CSF protein, CSF1 protein, Macrophage Colony Stimulating Factor 1 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of MCSF protein (His tag) (80R-1225)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 33-190 amino acids: MGSSHHHHHH SSGLVPRGSH MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ DVVTKPDCN
Expression System E.coli
Grade & Purity > 90% pure
Molecular Weight 20.7 kDa (179aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in . In 20mM Tris-HCl buffer, pH 8.0), 2mM DTT, 10% glycerol
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels < 1.0 EU per ug of protein (determined by LAL method )

General Information

Biological Significance Macrophage colony stimulating factor (M-CSF), as known as CSF-1, one of the hematopoietic growth factors that regulate the growth and differentiation of blood cells. This protein is produced by monocytes, granulocytes, endothelial cells, and fibroblasts. It stimulates the formation of macrophage colonies, enhances antibody-dependent, cell-mediated cytotoxicity by monocytes and macrophages, and inhibits bone resorption by osteoclasts. Recombinant human M-CSF, fused to His-tag at N-terminus, was expressed as insoluble protein aggregate in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. .

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Images

  • Coomassie Blue stained SDS-PAGE of MCSF protein (His tag) (80R-1225) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £176.28
Size: 100 ug
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