MDH1 protein (His tag) (80R-1427)

Purified recombinant Human MDH1 protein

Synonyms MDH-s protein, Malate dehydrogenase protein, MDHA protein, cytoplasmic, MOR2 protein, MDH-1, MDH 1 protein, MDH 1, MDH-1 protein, MDH1
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Prepare assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 100 mM Potassium phosphate, 0.13mM beta-nicotinamide adenine dinucleotide, reduced form, 0.25mM oxalacetic acid. 2. Add recombinant MDH protein with various concentrations (0.1ug, 0.5ug, 1ug) in assay buffer. 3. Mix by inversion, equilibrate to 1 DEg C, and monitor at A340nm until the value is constant using a spectrophotometer. 4. Record the increase at A340nm for approximately 2 minutes.

Specifications

Residues 1-334 amino acids: MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL DGVLMELQDC ALPLLKDVIATDKEDVAFKD LDVAILVGSM PRREGMERKD LLKANVKIFK SQGAALDKYA KKSVKVIVVG NPANTNCLTA SKSAPSIPKE NFSCLTRLDH NRAKAQIALK LGVTANDVKN VIIWGNHSST QYPDVNHAKV KLQGKEVGVY EALKDDSWLK GEFVTTVQQR GAAVIKARKL SSAMSAAKAI CDHVRDIWFG TPEGEFVSMG VISDGNSYGV PDDLLYSFPV VIKNKTWKFV EGLPINDFSR EKMDLTAKEL TEEKESAFEF LSSALEHHHH HH
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 37.4 kDa(342aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl Buffer, pH 8.0, containing 10% glycerol.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity Specific activity is > 8 units/mg, and is defined as the amount of enzyme that cleaves 1uM of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH7.5 and 25 DEG C.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance MDH1 is one of the two malate dehydrogenases. Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. In particular, MDH1 is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. This protein also regulates p53-dependent cell-cycle arrest and apoptosis in response to glucose deprivation. Recombinant human MDH1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography.

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Availability: In stock

Price: £204.32
Size: 100 ug
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