MECR protein (His tag) (80R-1370)
Purified recombinant Human MECR protein
|Synonyms||NBRF1 protein, Trans-2-enoyl-CoA reductase protein, FASN2B protein, NRBF1 protein, NRBF1 protein, Nuclear receptor binding factor 1, mitochondrial protein, NRBF 1 protein, CGI-63 protein, Homolog of yeast 2 enoyl thioester reductase protein, CGI 63 protein, HsNrbf1 protein, NBRF 1 protein|
Coomassie Blue stained SDS-PAGE of MECR protein (His tag) (80R-1370)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||54-373 amino acids: MGSSHHHHHH SSGLVPRGSH MPAKVVELKN LELAAVRGSD VRVKMLAAPI NPSDINMIQG NYGLLPELPA VGGNEGVAQV VAVGSNVTGL KPGDWVIPAN AGLGTWRTEA VFSEEALIQV PSDIPLQSAA TLGVNPCTAY RMLMDFEQLQ PGDSVIQNAS NSGVGQAVIQ IAAALGLRTI NVVRDRPDIQ KLSDRLKSLG AEHVITEEEL RRPEMKNFFK DMPQPRLALN CVGGKSSTEL LRQLARGGTM VTYGGMAKQP VVASVSLLIF KDLKLRGFWL SQWKKDHSPD QFKELILTLC DLIRRGQLTA PACSQVPLQD YQSALEASMK PFISSKQILT M|
|Grade & Purity||> 90% pure|
|Molecular Weight||36.9 kDa (341aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 0.2M NaCl, 5mM DTT and 20% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||MECR(Mitochondrial trans-2-enoyl-CoA reductase), also known as NRBF1, catalyzes the reduction of trans-2-enoyl-CoA to acyl-CoA with chain length from C6 to C16 in an NADPH dependent manner with preference to medium chain length substrate. This protein has a role in the mitochondrial synthesis of fatty acids. Recombinant human MECR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques|
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