MGMT protein (His tag) (80R-1094)
Purified recombinant Human MGMT protein
|Synonyms||MGMT protein, MGC107020 protein, EC 22.214.171.124 protein, Agat protein, O-6-methylguanine-DNA methyltransferase protein, AI267024 protein, Methylated DNA protein cysteine methyltransferase protein, Methylguanine DNA methyltransferase protein, O 6 methylguanine DNA alkyltransferase., O-6-methylguanine-DNA methyltransferase 6 O methylguanine DNA methyltransferase protein, O-6-methylguanine-DNA-alkyltransferase protein, AGT protein|
Coomassie Blue stained SDS-PAGE of MGMT protein (His tag) (80R-1094)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-207 amino acids: MGSSHHHHHH SSGLVPRGSH MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA AVLGGPEPLM QCTAWLNAYF HQPEAIEEFP VPAFHHPVFQ QESFTRQVLW KLLKVVKFGE VISYQQLAAL AGNPKAARAV GGAMRGNPVP ILIPCHRVVC SSGAVGNYSG GLAVKEWLLA HEGHRLGKPG LGGSSGLAGA WLKGAGATSG SPPAGRN|
|Grade & Purity||> 95% pure|
|Molecular Weight||23.8 kDa (227 aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 7.5, containing 1mM DTT, and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Endotoxin Levels||< 1.0 EU per ug of protein (determined by LAL method)|
|Biological Significance||O-6-methylguanine-DNA methyltransferase (MGMT) is an enzyme that repairs O-6-methylguanine, a mutagenic DNA base damaged by endogenous and environmental alkylating agents and is involved in the cellular defense against the biological effects of O-6-methylguanine in DNA. MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. There are few reports that abnormal MGMT expression correlates with the prognosis in human solid cancers. Recombinant MGMT, fused to His-tag at N-terminus, was expressed in E. coli and was purified by conventional chromatography techniques.|
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