MGMT protein (His tag) (80R-1094)

Purified recombinant Human MGMT protein

Synonyms MGMT protein, MGC107020 protein, EC 2.1.1.63 protein, Agat protein, O-6-methylguanine-DNA methyltransferase protein, AI267024 protein, Methylated DNA protein cysteine methyltransferase protein, Methylguanine DNA methyltransferase protein, O 6 methylguanine DNA alkyltransferase., O-6-methylguanine-DNA methyltransferase 6 O methylguanine DNA methyltransferase protein, O-6-methylguanine-DNA-alkyltransferase protein, AGT protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of MGMT protein (His tag) (80R-1094)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-207 amino acids: MGSSHHHHHH SSGLVPRGSH MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA AVLGGPEPLM QCTAWLNAYF HQPEAIEEFP VPAFHHPVFQ QESFTRQVLW KLLKVVKFGE VISYQQLAAL AGNPKAARAV GGAMRGNPVP ILIPCHRVVC SSGAVGNYSG GLAVKEWLLA HEGHRLGKPG LGGSSGLAGA WLKGAGATSG SPPAGRN
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 23.8 kDa (227 aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 7.5, containing 1mM DTT, and 10% glycerol.
Concentration 0.5 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels < 1.0 EU per ug of protein (determined by LAL method)

General Information

Biological Significance O-6-methylguanine-DNA methyltransferase (MGMT) is an enzyme that repairs O-6-methylguanine, a mutagenic DNA base damaged by endogenous and environmental alkylating agents and is involved in the cellular defense against the biological effects of O-6-methylguanine in DNA. MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. There are few reports that abnormal MGMT expression correlates with the prognosis in human solid cancers. Recombinant MGMT, fused to His-tag at N-terminus, was expressed in E. coli and was purified by conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of MGMT protein (His tag) (80R-1094) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £272.43
Size: 100 ug
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