MIF protein (His tag) (80R-1007)

Purified recombinant Human MIF protein

Synonyms Phenylpyruvate tautomerase protein, Macrophage migration inhibitory factor Glycosylation inhibiting factor protein, , MIF protein protein, Macrophage migration inhibitory factor protein, Glycosylation-inhibiting factor protein, MIF protein, EC 5.3.2.1 protein, GLIF protein, GIF protein, MMIF protein, MIF (1-114aa) protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of MIF protein (His tag) (80R-1007)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMPMF IVNTNVPRAS VPDGFLSELT QQLAQATGKP PQYIAVHVVP DQLMAFGGSS EPCALCSLHS IGKIGGAQNR SYSKLLCGLL AERLRISPDR VYINYYDMNA ANVGWNNSTF A
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 16.6 kDa (151 aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in PBS, pH 7.4.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels < 1.0 EU per ug of protein (determined by LAL method)

General Information

Biological Significance The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration. Recombinant human MIF, fused to His-tag at N-terminus, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of MIF  protein (His tag) (80R-1007) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £144.23
Size: 100 ug
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