Mouse IgG (HRP) (65C-CH1003)
Purified Mouse IgG HRP conjugate
|Synonyms||Mouse Immunoglobulin G (HRP)|
SDS-PAGE analysis of Mouse IgG (HRP) (65C-CH1003)
Lane 1: 5 uL MW Marker. Lane 2: Reduced Mouse IgG Whole Molecule Peroxidase Conjugated. Lane 3: Reduced Mouse F(c) Fragment. Lane 4: Reduced Mouse F(ab) Fragment. Lane 5: Mouse IgM Kappa Myeloma Protein. Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
|Method of Purification||Mouse IgG (HRP) was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.|
|Form & Buffer||Lyophilized from 0.01M Na3PO4, pH 7.4, with 0.14M NaCl, 10 mg/ml BSA, and 0.01% gentamicin sulfate. Immunoglobulin and protease free.|
Storage & Safety
|Storage||Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains ? and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer.|
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