MRPL49 antibody (70R-9977)
Affinity purified rabbit polyclonal MRPL49 antibody
|Synonyms||Polyclonal MRPL49 antibody, Anti-MRPL49 antibody, MRPL-49 antibody, NOF1 antibody, C11orf4 antibody, L49mt antibody, MRPL 49 antibody, NOF antibody, MRPL-49, mitochondrial ribosomal protein L49 antibody, MRPL 49, MGC10656 antibody, MRPL49|
|Immunogen||MRPL49 antibody was raised in rabbit using the N terminal of MRPL49 as the immunogen|
|Assay Information||MRPL49 Blocking Peptide, catalog no. 33R-4069, is also available for use as a blocking control in assays to test for specificity of this MRPL49 antibody|
Western Blot analysis using MRPL49 antibody (70R-9977)
Western Blot showing MRPL49 antibody used at a concentration of 1-2 ug/ml to detect its target protein.
|Form & Buffer||Lyophilized powder. Add 50 ul of distilled water. Final antibody concentration is 1 mg/ml in PBS buffer.|
Usage & Assay Information
|Usage Recommendations||WB: 0.2-1 ug/ml|
Storage & Safety
|Storage||Store at 4 deg C, following reconstitution, aliquot and store at -20 deg C. Avoid repeated freeze-thaw cycles.|
|Biological Significance||Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene and the gene for the HRD1 protein use in their respective 3' UTRs some of the same genomic sequence. Pseudogenes corresponding to this gene are found on chromosomes 5q and 8p.|
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