MYL2 protein (His tag) (80R-1085)
Purified recombinant Human MYL2 protein
|Synonyms||Myosin light polypeptide 2 regulatory cardiac slow protein, Myosin regulatory light chain 2 ventricular cardiac muscle isoform protein, , DKFZp779C0562 protein, Myosin light chain 2 regulatory cardiac slow protein, CMH10 protein, MYL-2, Slow cardiac myosin regulatory light chain 2 protein, MYL-2 protein, MLC2 protein, Cardiac ventricular myosin light chain 2 protein, Regulatory light chain of myosin protein, MYL2 protein, Myosin regulatory light chain 2 ventricular/cardiac muscle isoform protein, MYL 2 protein, RLC of myosin protein, MYL 2, Slow cardiac myosin regulatory light chain 2 Cardiac myosin light chain-2 protein, MLC 2v protein, MYL2, MYL 2 protein|
Coomassie Blue stained SDS-PAGE of MYL2 protein (His tag) (80R-1085)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-166 amino acids: MGSSHHHHHH SSGLVPRGSH MAPKKAKKRA GGANSNVFSM FEQTQIQEFK EAFTIMDQNR DGFIDKNDLR DTFAALGRVN VKNEEIDEMI KEAPGPINFT VFLTMFGEKL KGADPEETIL NAFKVFDPEG KGVLKADYVR EMLTTQAERF SKEEVDQMFA AFPPDVTGNL DYKNLVHIIT HGEEKD|
|Grade & Purity||> 95% pure|
|Molecular Weight||20.9 kDa (186aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris, pH 8.0, containing 20% glycerol, and 5mM CaCl2.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Myosin, light chain 2 (MYL2) encodes the regulatory light chain associated with cardiac myosin beta heavy chain. It is an important protein involved in the regulation of myosin ATPase activity in smooth muscle and Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. Recombinant human MYL2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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