Na,K-ATPase alpha 3 antibody (10R-S113a)

Mouse monoclonal Na,K-ATPase alpha 3 antibody

Synonyms Monoclonal Na,K-ATPase alpha 3 antibody, Sodium/ Potassium ATPase alpha-3 antibody, K-ATPase alpha 3 antibody, Anti Na, Na, ATPase Na/K alpha 3, ATPase Na/K alpha -3, ATPase Na/K alpha -3 antibody, ATPase Na/K alpha 3 antibody, ATPase Na/K alpha 3, K-ATPase alpha 3 fusion protein antibody
Specificity Dog
Cross Reactivity Dog, guinea pig, human, non-human primate, rabbit, rat, sheep
Applications IF, IHC-F, WB
Immunogen Na,K-ATPase alpha 3 antibody was raised in mouse using canine cardiac microsomes as the immunogen.

Images

Immunohistochemical staining using Na,K-ATPase alpha 3 antibody (10R-S113a)

Immunohistochemistry was performed on normal deparaffinized Human colon tissue tissues. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-3 (MA3-915) or without primary antibody (negative control) overnight at 4ðC in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Specifications

Host Mouse
Clone XVIF9-G10
Isotype IgG1
Form & Buffer PBS-diluted ascites containing 0.05% NaN3.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations IHC-F: 3 ug/ml, IF: 5 ug/ml, WB: 1 ug/ml

Storage & Safety

Storage Aliquot and store at -20 deg C. Avoid repeated freeze/thaw cycles.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane.

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Images

  • Immunohistochemical staining using Na,K-ATPase alpha 3 antibody (10R-S113a) | Immunohistochemistry was performed on normal deparaffinized Human colon tissue tissues. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-3 (MA3-915) or without primary antibody (negative control) overnight at 4ðC in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Availability: In stock

Price: £500.78
Size: 100 ul
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