NDE1 protein (His tag) (80R-1784)
Purified recombinant Human NDE1 protein
|Synonyms||NDE-1 protein, NDE-1, Nuclear distribution protein nudE homolog 1 protein, NDE 1 protein, NUDE protein, FLJ20101 protein, NUDE protein, HOM-TES-87 protein, nudE nuclear distribution gene E homolog 1 (A. nidulans) protein, NDE1, NDE 1|
Coomassie Blue stained SDS-PAGE of NDE1 protein (His tag) (80R-1784)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-335 amino acids: MGSSHHHHHH SSGLVPRGSH MEDSGKTFSS EEEEANYWKD LAMTYKQRAE NTQEELREFQ EGSREYEAEL ETQLQQIETR NRDLLSENNR LRMELETIKE KFEVQHSEGY RQISALEDDL AQTKAIKDQL QKYIRELEQA NDDLERAKRA TIMSLEDFEQ RLNQAIERNA FLESELDEKE NLLESVQRLK DEARDLRQEL AVQQKQEKPR TPMPSSVEAE RTDTAVQATG SVPSTPIAHR GPSSSLNTPG SFRRGLDDST GGTPLTPAAR ISALNIVGDL LRKVGALESK LASCRNLVYD QSPNRTGGPA SGRSSKNRDG GERRPSSTSV PLGDKGLDTS CRWLSKSTTR SSSSC|
|Grade & Purity||> 85% pure|
|Molecular Weight||39.9 kDa (355aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 5mM DTT and 0.2M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||NDE1 is a cytoplasmic protein belonging to the nudE protein family. Phosphorylated during mitosis, it is essential for the formation and function of the mitotic spindle in M phase and functions to regulate the Dynein-mediated transport of kinetochore proteins, as well as centrosomes duplication during interphase. NDE1 is thought to interact with NDEL1, LIS1 and Dynein IC1/2, cytosolic in a signaling pathway that regulates the formation of neurons and is fundamental to the development of the cerebral cortex. Recombinant human NDE1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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