NDRG1 protein (His tag) (80R-1193)

Purified recombinant Human NDRG1 protein

Synonyms ndr1 protein, Protein nickel specific induction protein protein, Reducing agents and tunicamycin responsive protein protein, Protein regulated by oxygen 1 protein, N-myc downstream regulated 1 TDD5 protein, N myc downstream regulated gene 1 protein protein, Human mRNA for RTP complete cds protein, Differentiation related gene1 protein protein, Nmyc downstream regulated gene1 protein protein, NDRG 1 protein, RTP protein, reducin protein, NDRG 1 protein, NDRG1 protein protein, RIT42 protein, cmt4d protein, 42 kDa protein, reduced in tumor protein, NDRG1 protein, Nickel specific induction protein Cap43 protein, CAP43 protein, TARG1 protein, CMT4D protein, anti-GC4 protein, proxy1 protein, NDRG-1, Ndr 1 protein, HMSNL protein, NDRG 1, Protein regulated by oxygen1 protein, NDRG1, nmsl protein, DRG1 protein, Nmyc downstream regulated protein, Nmyc downstream regulated gene1 protein, Drg 1 protein, tdds protein, N myc downstream regulated gene 1 protein, hmsnl protein, TDD5 protein, NMSL protein, tunicamycin-responsive protein, PROXY1 protein, GC4 protein, NDRG-1 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Cell line: MCF7 (Human breast adenocarcinoma cell) 2. Maintenance Condition: RPMI 1640 containing 10% FBS 3. Assay medium: serum free RPMI 1640 4. Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) 5. Incubation time : 40 hrs (after sample treatment) 6. Concentration range: 0.19ng/ml - 50ng/ml 7. Detection method : BrdU assay

Images

Coomassie Blue stained SDS-PAGE of NDRG1 protein (His tag) (80R-1193)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-394 amino acids: MSREMQDVDL AEVKPLVEKG ETITGLLQEF DVQEQDIETL HGSVHVTLCG TPKGNRPVIL TYHDIGMNHK TCYNPLFNYE DMQEITQHFA VCHVDAPGQQ DGAASFPAGY MYPSMDQLAE MLPGVLQQFG LKSIIGMGTG AGAYILTRFA LNNPEMVEGL VLINVNPCAE GWMDWAASKI SGWTQALPDM VVSHLFGKEE MQSNVEVVHT YRQHIVNDMN PGNLHLFINA YNSRRDLEIE RPMPGTHTVT LQCPALLVVG DSSPAVDAVV ECNSKLDPTK TTLLKMADCG GLPQISQPAK LAEAFKYFVQ GMGYMPSASM TRLMRSRTAS GSSVTSLDGT RSRSHTSEGT RSRSHTSEGT RSRSHTSEGA HLDITPNSGA AGNSAGPKSM EVSCLEHHHH HH
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 43.9 kDa (402aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 0.1 mM PMSF, and 10% glycerol.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity The ED50 for this effect is 0.5 - 1.5 ng/ml. Measured in a cell proliferation assay using MCF7 cell.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance N-myc downstream regulated gene (NDRG)-1 is one of 4 members of the NDRG alpha/beta-hydrolase family. This protein is classified in databases as a tumor suppressor and heavy metal-response protein. Its functions include cell-cycle regulation, cellular differentiation, apoptosis, hypoxia response and metal-ion sensing. Mutation in the NDRG1 gene has been reported to be causative for hereditary motor and sensory neuropathy-Lom (HMSNL), a severe autosomal recessive form of Charcot-Marie-Tooth (CMT) disease. Recombinant human NDRG1, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Coomassie Blue stained SDS-PAGE of NDRG1 protein (His tag) (80R-1193) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £144.23
Size: 100 ug
OR
Shipping
View Our Distributors