NEDD8 protein (His tag) (80R-1104)
Purified recombinant Human NEDD8 protein
|Synonyms||Rub1 protein, developmentally down-regulated 8 MGC104393 protein, MGC125896 protein, Neddylin protein, NEDD 8, NEDD-8, Ubiquitin like protein Nedd8., Neural precursor cell expressed developmentally down regulated gene 8 protein, Neural precursor cell expressed protein, NED8 protein, NEDD8, NEDD 8 protein, MGC125897 protein, NEDD-8 protein, Neural precursor cell expressed developmentally down regulated 8 protein, 0NEDD 8 protein, Neddylin protein, Ubiquitin-like protein Nedd8 protein, Ubiquitin like protein Nedd 8 protein|
Coomassie Blue stained SDS-PAGE of NEDD8 protein (His tag) (80R-1104)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-76 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMLI KVKTLTGKEI EIDIEPTDKV ERIKERVEEK EGIPPQQQRL IYSGKQMNDE KTAADYKILG GSVLHLVLAL RGG|
|Grade & Purity||> 95% pure|
|Molecular Weight||12.8 kDa (113 aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 0.5mM DTT, 50mM NaCl, and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Endotoxin Levels||< 1.0 EU per ug of protein (determined by LAL method)|
|Biological Significance||NEDD8 belongs to the ubiquitin family and human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The NEDD8 system is essential for the regulation of protein degradation pathways involved in cell cycle progression, morphogenesis and tumorigenesis. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Recombinant human NEDD8 protein was expressed in E. coli and purified by using conventional chromatography techniques, after refolding of the isolated inclusion body in renaturation buffer.|
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