NHP2 protein (His tag) (80R-1624)
Purified recombinant Human NHP2 protein
|Synonyms||NHP2, NHP 2, NHP-2 protein, NHP 2 protein, NOLA2, NHP-2, H/ACA ribonucleoprotein complex subunit 2 isoform a protein, NHP2P protein|
Coomassie Blue stained SDS-PAGE of NHP2 protein (His tag) (80R-1624)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-153 amino acids: MGSSHHHHHH SSGLVPRGSH MTKIKADPDG PEAQAEACSG ERTYQELLVN QNPIAQPLAS RRLTRKLYKC IKKAVKQKQI RRGVKEVQKF VNKGEKGIMV LAGDTLPIEV YCHLPVMCED RNLPYVYIPS KTDLGAAAGS KRPTCVIMVK PHEEYQEAYD ECLEEVQSLP LPL|
|Grade & Purity||> 90% pure|
|Molecular Weight||19.3 kDa (173aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 0.1M NaCl and 1mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||NHP2, also called NOLA2, forms a small ribonucleoprotein particle with GAR1(NOLA1), Nop10 for the isomerization of uridine to pseudouridine. This protein is involved in various aspects of rRNA processing and modification. It localizes to the dense fibrillar component of the nucleolus and in nuclear Cajal bodies. Recombinant human NHP2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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