NNMT protein (His tag) (80R-1151)

Purified recombinant Human NNMT protein

Synonyms Nicotinamide N-methyltransferase protein, Nicotinamide N-methyltransferase EC 2.1.1.1 Nicotinamide N methyltransferase., NNMT protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of NNMT protein (His tag) (80R-1151)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-264 amino acids: MGSSHHHHHH SSGLVPRGSH MESGFTSKDT YLSHFNPRDY LEKYYKFGSR HSAESQILKH LLKNLFKIFC LDGVKGDLLI DIGSGPTIYQ LLSACESFKE IVVTDYSDQN LQELEKWLKK EPEAFDWSPV VTYVCDLEGN RVKGPEKEEK LRQAVKQVLK CDVTQSQPLG AVPLPPADCV LSTLCLDAAC PDLPTYCRAL RNLGSLLKPG GFLVIMDALK SSYYMIGEQK FSSLPLGREA VEAAVKEAGY TIEWFEVISQ SYSSTMANNE GLFSLVARKL SRPL
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 31.7 kDa (284aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance NNMT(Nicotinamide N-methyltransferase) belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. It is predominantly expressed in the liver, and a lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. NNMT catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. Recombinant human NNMT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of NNMT protein (His tag) (80R-1151) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £204.32
Size: 100 ug
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