Noggin protein (30R-AN040)

Purified recombinant Human Noggin protein

Synonyms SYM1 protein, NOG protein, SYM 1 protein, SYNS 1 protein, Symphalangism 1 (proximal) protein, Synostoses (multiple) syndrome 1 protein, SYNS1 protein
Species Human
Protein Type Recombinant
Applications User optimized

Specifications

Residues Region of Noggin protein corresponding to amino acids QHYLHIRPAP SDNLPLVDLI EHPDPIFDPK EKDLNETLLR SLLGGHYDPG FMATSPPEDR PGGGGGAAGG AEDLAELDQL LRQRPSGAMP SEIKGLEFSE GLAQGKKQRL SKKLRRKLQM WLWSQTFCPV LYAWNDLGSR FWPRYVKVGS CFSKRSCSVP EGMVCKPSKS VHLTVLRWRC QRRGGQRCGW IPIQYPIISE CKCSC.
Expression System 293 cells
Grade & Purity > 98% pure
Molecular Weight 46 kDa
Form & Buffer Supplied lyophilized with no additives. Reconstitute in water to a concentration of 0.1 - 1.0 mg/ml.

Usage & Assay Information

Bioactivity Determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC chondrogenic cells. The expected ED50 for this effect is 2.0-3.0 ng/ml of Noggin.

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels Endotoxin level is less than 0.1 ng per ug (1 EU/ug).

General Information

Biological Significance Noggin belongs to a group of diffusible proteins which bind to ligands of the TGF beta family and regulate their activity by inhibiting their access to signaling receptors. The interplay between TGF beta ligands and their natural antagonists has major biological significance during development processes, in which cellular response can vary considerably depending upon the local concentration of the signaling molecule. Noggin was originally identified as a BMP-4 antagonist whose action is critical for proper formation of the head and other dorsal structures. Consequently, Noggin has been shown to modulate the activities of other BMPs including BMP-2, -7, -13, and -14. Targeted deletion of Noggin in mice results in prenatal death and recessive phenotype displaying a severely malformed skeletal system.

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Availability: In stock

Price: £180.28
Size: 20 ug
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