NONO antibody (70R-1315)

Rabbit polyclonal NONO antibody raised against the N terminal of NONO

Synonyms Polyclonal NONO antibody, Anti-NONO antibody, Non-Pou Domain Containing Octamer-Binding antibody
Specificity NONO antibody was raised against the N terminal of NONO
Cross Reactivity Human,Mouse,Rat,Dog
Applications WB
Immunogen NONO antibody was raised using the N terminal of NONO corresponding to a region with amino acids KQNHTPRKHHQHHHQQQHHQQQQQQPPPPPIPANGQQASSQNEGLTIDLK
Assay Information NONO Blocking Peptide, catalog no. 33R-4610, is also available for use as a blocking control in assays to test for specificity of this NONO antibody

Images

Western Blot analysis using NONO antibody (70R-1315)

NONO antibody (70R-1315) used at 1.25 ug/ml to detect target protein.

Specifications

Host Rabbit
Method of Purification Total IgG Protein A purified
Molecular Weight 52 kDa (MW of target protein)
Form & Buffer Lyophilized powder. Add 100ul distilled water for a 1mg/ml concentration of NONO antibody in PBS
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1.25 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for short periods. For longer periods of storage, store at -20 deg C. Avoid repeat freeze-thaw cycles.

General Information

Biological Significance NONO is DNA- and RNA binding protein, involved in several nuclear processes. It binds the conventional octamer sequence in double stranded DNA. It also binds single-stranded DNA and RNA at a site independent of the duplex site. It is involved in pre-mRNA splicing and interacts with U5 snRNA. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs, be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1 and be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription.

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Images

  • Western Blot analysis using NONO antibody (70R-1315) | NONO antibody (70R-1315) used at 1.25 ug/ml to detect target protein.

Availability: In stock

Price: £220.34
Size: 100 ug
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