NUDT1 protein (His tag) (80R-1504)
Purified recombinant Human NUDT1 protein
|Synonyms||MTH1 protein, NUDT 1 protein, 7 protein, 8-dihydro-8-oxoguanine triphosphatase, NUDT-1, 8-oxo-dGTPase protein, NUDT 1, NUDT1, NUDT-1 protein, Nudix motif 1 protein|
Coomassie Blue stained SDS-PAGE of NUDT1 protein (His tag) (80R-1504)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-156 amino acids: MGSSHHHHHH SSGLVPRGSH MGASRLYTLV LVLQPQRVLL GMKKRGFGAG RWNGFGGKVQ EGETIEDGAR RELQEESGLT VDALHKVGQI VFEFVGEPEL MDVHVFCTDS IQGTPVESDE MRPCWFQLDQ IPFKDMWPDD SYWFPLLLQK KKFHGYFKFQ GQDTILDYTL REVDTV|
|Grade & Purity||> 95% pure|
|Molecular Weight||20.1 kDa (176aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 2mM DTT and 100mM NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||NUDT1 is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation of 8-oxo-dGTP into DNA thus preventing A:T to C:G transversions. It is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. And this protein expressed at much higher levels in proliferating cells than in resting cells. Recombinant human NUDT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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