NUTF2 protein (His tag) (80R-1358)
Purified recombinant Human NUTF2 protein
|Synonyms||PP 15, NUTF2, NTF2 protein, Nuclear transport factor 2 protein, NUTF-2 protein, Placental protein 15 protein, NUTF 2 protein, NUTF 2, PP15 protein, NUTF-2, NUTF 2 protein|
Coomassie Blue stained SDS-PAGE of NUTF2 protein (His tag) (80R-1358)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-127 amino acids: MGSSHHHHHH SSGLVPRGSH MGDKPIWEQI GSSFIQHYYQ LFDNDRTQLG AIYIDASCLT WEGQQFQGKA AIVEKLSSLP FQKIQHSITA QDHQPTPDSC IISMVVGQLK ADEDPIMGFH QMFLLKNIND AWVCTNDMFR LALHNFG|
|Grade & Purity||> 95% pure|
|Molecular Weight||16.6 kDa(147aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0 containing 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||NUTF2 is a cytosolic factor that facilitates protein transport into the nucleus. It interacts with the nuclear pore complex glycoprotein p62 and Ran. This protein acts at a relatively late stage of nuclear protein import, subsequent to the initial docking of nuclear import ligand at the nuclear envelope. It binds directly to RanGDP and may modulate the steady-state distribution of Ran. Recombinant human NUTF2, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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