Osteopontin protein (His tag) (80R-1119)
Purified recombinant Human Osteopontin protein
|Synonyms||ETA1 protein, BNSP protein, , MGC110940 protein, ETA-1 protein, Uropontin protein, OPN protein, SPP 1 protein, Bone sialoprotein 1 protein, BSP I.Early T lymphocyte activation 1 protein, Nephropontin protein, SPP-1 protein, BSPI protein, SPP1 protein, Secreted phosphoprotein 1 protein, ETA 1 protein, Urinary stone protein protein|
Coomassie Blue stained SDS-PAGE of Osteopontin protein (His tag) (80R-1119)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||17-314 amino acids: MGSSHHHHHH SSGLVPRGSH RSMIPVKQAD SGSSEEKQLY NKYPDAVATW LNPDPSQKQN LLAPQNAVSS EETNDFKQET LPSKSNESHD HMDDMDDEDD DDHVDSQDSI DSNDSDDVDD TDDSHQSDES HHSDESDELV TDFPTDLPAT EVFTPVVPTV DTYDGRGDSV VYGLRSKSKK FRRPDIQYPD ATDEDITSHM ESEELNGAYK AIPVAQDLNA PSDWDSRGKD SYETSQLDDQ SAETHSHKQS RLYKRKANDE SNEHSDVIDS QELSKVSREF HSHEFHSHED MLVVDPKSKE EDKHLKFRIS HELDSASSEV N|
|Grade & Purity||> 90% pure|
|Molecular Weight||36.2 kDa (321aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 7.5, containing 1mM DTT, 10% glycerol, and 2mM EDTA.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Osteopontin (OPN), also known as Secreted phosphoprotein 1 (SPP1), is a human gene product which is conserved in other species. This protein is a glycoprotein that was first identified in osteoblasts and plays an important role in bone remodeling, immune functions in fibroblasts, macrophages, and lymphocytes during inflammation and wound healing. Recombinant human Osteopontin protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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