OSTF1 protein (His tag) (80R-1108)
Purified recombinant Human OSTF1 protein
|Synonyms||OSF protein, OSTF 1 protein, OSTF1, Osteoclast stimulating factor 1 '', OSTF-1, OSTF 1, OSTF-1 protein, SH3P2 protein|
Coomassie Blue stained SDS-PAGE of OSTF1 protein (His tag) (80R-1108)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-217 amino acids: MSKPPPKPVK PGEGGQVKVF RALYTFEPRT PDELYFEEGD IIYITDMSDT NWWKGTSKGR TGLIPSNYVA EQAESIDNPL HEAAKRGNLS WLRECLDNRV GVNGLDKAGS TALYWACHGG HKDIVEMLFT QPNIELNQQN KLGDTALHAA AWKGYADIVQ LFLAKGARTD LRNIEKKLAF DMATNAACAS LLKKKQGTDA VRTLSNAEDY LDDEDSDLEH HHHHH|
|Grade & Purity||> 95% pure|
|Molecular Weight||25.1 kDa (225 aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 0.5mM DTT, and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Osteoclast-stimulating factor-1 (OSTF1) is an intracellular protein produced by osteoclasts that indirectly induces osteoclast (OCL) formation and bone resorption. OSTF1 is highly expressed in OCL and contains structural domains such as SH3 and ankyrin repeats, which are potentially involved in protein-protein interactions. OSTF1 is ubiquitously expressed analogous to other regulators of OCL activity such as c-Src. Recombinant human OSTF1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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