PCNA antibody (biotin) (61R-P152aBT)

Mouse monoclonal PCNA antibody (biotin)

Synonyms Monoclonal PCNA antibody, Anti-PCNA antibody, PCNA antibody, Prostate Cell Nuclear Antigen antibody, Anti PCNA antibody, Prostate Cell Nuclear Antigen antibody
Specificity Rat
Cross Reactivity Human, mouse, insect, yeast
Applications FC, IHC-F, IHC-P, IP, WB
Immunogen PCNA antibody (biotin) was raised in mouse using recombinant rat proliferating cell nuclear antigen protein as the immunogen.

Images

Immunohistochemical staining using PCNA antibody (biotin) (61R-P152aBT)

Formalin-fixed, paraffin-embedded human small intestine stained with PCNA antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of proliferating cell

Specifications

Host Mouse
Clone PC10
Isotype IgG2a kappa
Method of Purification PCNA antibody (biotin) was purified by Protein G chromatography.
Molecular Weight 36 kDa (predicted detection band MW)
Tag/Conjugate Biotin
Form & Buffer Purified from ascites fluid by protein G chromatography and supplied in 10mM PBS, pH 7.4.
Concentration Batch dependent - please inquire should you have specific requirements

Usage & Assay Information

Usage Recommendations IHC-F: 1:200-1:400, IHC-P: 1:200-1:400, IP: 2 ug/ml protein lysate, WB: 1:50

Storage & Safety

Storage Store at 4 deg C.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling.

General Information

Biological Significance Expression of proliferating cell nuclear antigen (PCNA) or cyclin or polymerase delta auxiliary protein is elevated in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. Its level correlates directly with rates of cellular proliferation and DNA synthesis. PCNA/cyclin may act as an auxiliary protein of DNA polymerase-delta to play a fundamental role in the initiation of cell proliferation.

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Images

  • Immunohistochemical staining using PCNA antibody (biotin) (61R-P152aBT) | Formalin-fixed, paraffin-embedded human small intestine stained with PCNA antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of proliferating cell
  • Immunohistochemical staining using PCNA antibody (biotin) (61R-P152aBT) | Formalin-fixed, paraffin-embedded rat breast cancer stained with PCNA antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.

Availability: In stock

Price: £412.64
Size: 100 ul
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