PCNP protein (His tag) (80R-1932)
Purified recombinant PCNP protein
|Synonyms||PEST proteolytic signal containing nuclear protein. protein, DKFZp781I24156 protein|
|Assay Information||PCNP protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.|
Coomassie Blue stained SDS-PAGE of PCNP protein (His tag) (80R-1932)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-178 amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMADGKA GDEKPEKSQR AGAAGGPEEE AEKPVKTKTV SSSNGGESSS RSAEKRSAEE EAADLPTKPT KISKFGFAIG SQTTKKASAI SIKLGSSKPK ETVPTLAPKT LSVAAAFNED EDSEPEEMPP EAKMRMKNIG RDTPTSAGPN SFNKGKHGFS DNQKLWERNI KSHLGNVHDQ DN|
|Grade & Purity||> 95% pure|
|Molecular Weight||21.5 kDa|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, with 10% glycerol and 1mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Aliquot and store at -20 deg C or -70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||PCNP (PEST proteolytic signal containing nuclear protein) is a nuclear protein implied to play a role in cell cycle regulation and tumorigenesis. This protein is ubiquitinated post-translationally by NIRF, an ubiquitin ligase. PCNP exsists as three isoforms produced by alternative splicing events and may be involved in cell cycle regulation. Recombinant human PCNP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
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