PDGF BB protein (30R-AP051)

Purified recombinant Human PDGF BB protein

Synonyms Osteosarcoma-derived Growth Factor protein, Glioma-derived growth factor protein, Platelet-Derived Growth Factor-BB protein, GDGF protein, ODGF protein
Species Human
Protein Type Recombinant
Applications User optimized

Specifications

Residues Region of PDGF BB protein corresponding to amino acids MSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT.
Expression System E.coli
Grade & Purity > 98% pure
Molecular Weight 28.7 kDa
Form & Buffer Supplied as a lyophilized powder.

Usage & Assay Information

Bioactivity Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 1.0-2.0 ng/ml.

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels Endotoxin level is less than 0.1 ng per ug (1 EU/ug).

General Information

Biological Significance PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet a-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-a and PDGFR-beta. PDGFR-a is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB.

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Availability: In stock

Price: £180.28
Size: 10 ug
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