PDK1 antibody (10R-1157)

Mouse monoclonal PDK1 antibody

Synonyms Monoclonal PDK1 antibody, Anti-PDK1 antibody, isozyme 1 HGNC:8809 antibody, isozyme 1 antibody, Pyruvate dehydrogenase kinase isoform 1 antibody, Mitochondrial pyruvate dehydrogenase kinase isoenzyme1 antibody, Pyruvate dehydrogenase kinase antibody, PDK 1, PDK1, Pyruvate dehydrogenase kinase antibody, isoenzyme 1. antibody, Pyruvate dehydrogenase kinase antibody, PDK-1 antibody, PDK 1 antibody, PDK1 antibody, PDK-1
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, WB
Immunogen PDK1 antibody was raised in mouse using recombinant human PDK1 (29-436aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of PDK1 antibody (10R-1157)

The extracts of rat heart (60 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human PDK1 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone AT2D6
Isotype IgG1 kappa
Source Anti-human PDK1 antibody, clone AT2D6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PDK1 protein.
Method of Purification PDK1 antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1:250-1:1,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance PDK1 (pyruvate dehydrogenase kinase isoform 1) is involved in the regulation of enzymatic activity of mammalian pyruvate dehydrogenase (PDH) that is a part of a mitochondrial multienzyme complex to catalyze the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. PDK1 has been found to serve as an effective therapeutic target for inhibition of glioblastoma growth.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Western Blot analysis of PDK1 antibody (10R-1157) | The extracts of rat heart (60 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human PDK1 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: £212.33
Size: 100 ul
OR
Shipping
View Our Distributors