PEDF protein (His tag) (80R-1164)

Purified recombinant Human PEDF protein

Synonyms PIG 35 protein, PIG35 protein, SERPINF1 protein, Pigment epithelium-derived factor EPC 1 protein, Proliferation inducing protein 35 protein, SERPIN F1 protein, EPC-1 protein, EPC1 protein, Serpin peptidase inhibitor clade F member 1 protein, , SERPINF 1 protein, Pigment epithelium-derived factor protein, Pigment epithelium derived factor protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of PEDF protein (His tag) (80R-1164)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 20-418 amino acids: MGSSHHHHHH SSGLVPRGSH MQNPASPPEE GSPDPDSTGA LVEEEDPFFK VPVNKLAAAV SNFGYDLYRV RSSMSPTTNV LLSPLSVATA LSALSLGAEQ RTESIIHRAL YYDLISSPDI HGTYKELLDT VTAPQKNLKS ASRIVFEKKL RIKSSFVAPL EKSYGTRPRV LTGNPRLDLQ EINNWVQAQM KGKLARSTKE IPDEISILLL GVAHFKGQWV TKFDSRKTSL EDFYLDEERT VRVPMMSDPK AVLRYGLDSD LSCKIAQLPL TGSMSIIFFL PLKVTQNLTL IEESLTSEFI HDIDRELKTV QAVLTVPKLK LSYEGEVTKS LQEMKLQSLF DSPDFSKITG KPIKLTQVEH RAGFEWNEDG AGTTPSPGLQ PAHLTFPLDY HLNQPFIFVL RDTDTGALLF IGKILDPRGP
Expression System E.coli
Grade & Purity > 85% pure
Molecular Weight 46.7 kDa (420 aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol.
Concentration 0.5 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance PEDF, also known as SERPINF1, is an extracellular neurotrophic agent, first identified as a secreted product in conditioned medium from cultured fetal human retinal pigment epithelial (RPE) cells. It was reported that PEDF isolated from medium conditioned by human fetal RPE primary cultures promotes neurite outgrowth in cultured human retinoblastoma Y-79 cells. PEDF is also a member of the serpin family, a group of serine protease inhibitors. As PEDF does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. Recombinant PEDF protein containing a natural variant M72T was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of PEDF protein (His tag) (80R-1164) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £204.32
Size: 100 ug
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