Peroxiredoxin 6 protein (His tag) (80R-1174)

Purified recombinant Human Peroxiredoxin 6 protein

Synonyms 9430088D19Rik protein, PHGPx protein, Aop2 rs3 protein, Peroxiredoxin6 protein, Brp 12 protein, AA690119 protein, Peroxiredoxin -6 protein, , Peroxiredoxin6 protein, Acidic calcium independent phospholipase A2 protein, AOP2 protein, EC 1.11.1.7 protein, NSGPx protein, Liver 2D page spot 40 protein, EC 3.1.1 protein, ORF06 protein, Prdx6 rs3 protein, Lvtw 4 protein, 1-Cys protein, Red blood cells page spot 12 protein, GPx protein, PRX protein, PLA2 protein, mKIAA0106 protein, CP 3 protein, Peroxiredoxin 6, PRDX-6 protein, KIAA0106 protein, 1 Cys protein, 1 Cys peroxiredoxin protein, Ciliary body glutathione peroxidase protein, EC 1.11.1.15 protein, Antioxidant protein 2 protein, 1 cysPrx protein, Peroxiredoxin 6 protein, 24 kDa protein protein, p29 protein, OTTHUMP00000032693 protein, Peroxiredoxin 6, MGC46173 protein, Non selenium glutathione peroxidase protein, Peroxiredoxin -6, Phospholipase A2 lysosomal protein, NSGPx protein, PRDX6 protein, PRX protein, Ltw4 protein, Prdx5 protein, Thiol specific antioxidant protein protein, aiPLA2 protein, 1 Cys PRX protein, PRDX 6 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of Peroxiredoxin 6 protein (His tag) (80R-1174)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-224 amino acids: MGSSHHHHHH SSGLVPRGSH MPGGLLLGDV APNFEANTTV GRIRFHDFLG DSWGILFSHP RDFTPVCTTE LGRAAKLAPE FAKRNVKLIA LSIDSVEDHL AWSKDINAYN CEEPTEKLPF PIIDDRNREL AILLGMLDPA EKDEKGMPVT ARVVFVFGPD KKLKLSILYP ATTGRNFDEI LRVVISLQLT AEKRVATPVD WKDGDSVMVL PTIPEEEAKK LFPKGVFTKE LPSGKKYLRY TPQP
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 27.1 kDa (244aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity Specific activity is >2,000 pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for 1minute.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Peroxiredoxin 6, also known as PRDX6, is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell and can reduce H2O2 and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. Recombinant human peroxiredoxin 6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of Peroxiredoxin 6 protein (His tag) (80R-1174) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £204.32
Size: 100 ug
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