PNPO protein (His tag) (80R-1562)
Purified recombinant Human PNPO protein
|Synonyms||FLJ10535 protein, PDXPO, Pyridoxine-5'-phosphate oxidase protein|
Coomassie Blue stained SDS-PAGE of PNPO protein (His tag) (80R-1562)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||57-261 amino acids: MGSSHHHHHH SSGLVPRGSH MDPVKQFAAW FEEAVQCPDI GEANAMCLAT CTRDGKPSAR MLLLKGFGKD GFRFFTNFES RKGKELDSNP FASLVFYWEP LNRQVRVEGP VKKLPEEEAE CYFHSRPKSS QIGAVVSHQS SVIPDREYLR KKNEELEQLY QDQEVPKPKS WGGYVLYPQV MEFWQGQTNR LHDRIVFRRG LPTGDSPLGP MTHRGEEDWL YERLAP|
|Grade & Purity||> 90% pure|
|Molecular Weight||25.9 kDa (226aa) confirmed by MALDI-TOF (molecular size on SDS-PAGE will appear higher)|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 0.1M NaCl and 0.1mM PMSF.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||PNPO, also known as pyridoxine-5'-phosphate oxidase, is the rate-limiting enzyme in vitamin B6 synthesis. Vitamin B6, or pyridoxal 5-prime-phosphate (PLP), is critical for normal cellular function, and some cancer cells have notable differences in vitamin B6 metabolism compared to their normal counterparts. Recombinant human PNPO protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography|
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