PNPO protein (His tag) (80R-1562)

Purified recombinant Human PNPO protein

Synonyms FLJ10535 protein, PDXPO, Pyridoxine-5'-phosphate oxidase protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of PNPO protein (His tag) (80R-1562)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 57-261 amino acids: MGSSHHHHHH SSGLVPRGSH MDPVKQFAAW FEEAVQCPDI GEANAMCLAT CTRDGKPSAR MLLLKGFGKD GFRFFTNFES RKGKELDSNP FASLVFYWEP LNRQVRVEGP VKKLPEEEAE CYFHSRPKSS QIGAVVSHQS SVIPDREYLR KKNEELEQLY QDQEVPKPKS WGGYVLYPQV MEFWQGQTNR LHDRIVFRRG LPTGDSPLGP MTHRGEEDWL YERLAP
Expression System E.coli
Grade & Purity > 90% pure
Molecular Weight 25.9 kDa (226aa) confirmed by MALDI-TOF (molecular size on SDS-PAGE will appear higher)
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 0.1M NaCl and 0.1mM PMSF.
Concentration 0.5 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance PNPO, also known as pyridoxine-5'-phosphate oxidase, is the rate-limiting enzyme in vitamin B6 synthesis. Vitamin B6, or pyridoxal 5-prime-phosphate (PLP), is critical for normal cellular function, and some cancer cells have notable differences in vitamin B6 metabolism compared to their normal counterparts. Recombinant human PNPO protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography

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Images

  • Coomassie Blue stained SDS-PAGE of PNPO protein (His tag) (80R-1562) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £204.32
Size: 100 ug
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