PP2C alpha protein (His tag) (80R-1004)

Purified recombinant Human PP2C alpha protein

Synonyms PP2C alpha protein, PPC-2, FLJ42306 protein, MGC9201 protein, PPC 2, PP2C, ppm1a protein, Protein phosphatase 2C protein, protein phosphatase Mg2+/Mn2+ dependent 1A, Pyruvate dehydrogenase acetyl-transferring-phosphatase 1 protein, PDPC 1 protein, PPM2C protein, PDP 1 protein, PDP protein, PPC 2 protein, Protein phosphatase 1A (formerly 2C) protein, PP2CA protein, PPM1A protein, EC 3.1.3.43 protein, PP2Calpha protein, PPC-2 protein, Pyruvate dehydrogenase phosphatase catalytic subunit 1 protein, PP2C-alpha protein, PDP1 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information One unit will hydrolyze 1nanomole of p-nitrophenyl phosphatate(pNPP) per minute at pH 7.4 at 37 DEG C using 10mM of substrate

Images

Coomassie Blue stained SDS-PAGE of PP2C alpha protein (His tag) (80R-1004)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWILMGAF LDKPKMEKHN AQGQGNGLRY GLSSMQGWRV EMEDAHTAVI GLPSGLESWS FFAVYDGHAG SQVAKYCCEH LLDHITNNQD FKGSAGAPSV ENVKNGIRTG FLEIDEHMRV MSEKKHGADR SGSTAVGVLI SPQHTYFINC GDSRGLLCRN RKVHFFTQDH KPSNPLEKER IQNAGGSVMI QRVNGSLAVS RALGDFDYKC VHGKGPTEQL VSPEPEVHDI ERSEEDDQFI ILACDGIWDV MGNEELCDFV RSRLEVTDDL EKVCNEVVDT CLYKGSRDNM SVILICFPNA PKVSPEAVKK EAELDKYLEC RVEEIIKKQG EGVPDLVHVM RTLASENIPS LPPGGELASK RNVIEAVYNR LNPYKNDDTD STSTDDMW
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 46.6 kDa (418 aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 10mM Tris-HCl, pH7.5, containing 50mM NaCl, 2mM DTT, 1mM MnCl, and 20% glycerol.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity >8,000 units/mg of PP2C alpha

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels < 1.0 EU per ug of protein (determined by LAL method)

General Information

Biological Significance Protein phosphatase 2C(PP2Calpha) is a Mn ?+ - or Mg ?+ -dependent protein serine/threonine phosphatase that is essential for regulating cellular stress response in eukaryotes. The protein coding region of PP2Calpha(amino acids 1-382) was cloned into an E. coli expression vector(BamHI/Hind? site). PP2Calpha was overexpressed in E. coli as a soluble His-tag fusion protein, and it was purified by conventional column chromatographic techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of PP2C alpha protein (His tag) (80R-1004) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £144.23
Size: 100 ug
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