Profilin4 protein (His tag) (80R-1277)
Purified recombinant Human Profilin4 protein
|Synonyms||Profilin IV protein, Profilin-4 protein, Profilin family member 4., Profilin4, PFN4 protein, Profilin-4, Profilin 4, Profilin 4 protein|
Coomassie Blue stained SDS-PAGE of Profilin4 protein (His tag) (80R-1277)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-129 amino acids: MGSSHHHHHH SSGLVPRGSH MSHLQSLLLD TLLGTKHVDS AALIKIQERS LCVASPGFNV TPSDVRTLVN GFAKNPLQAR REGLYFKGKD YRCVRADEYS LYAKNENTGV VVVKTHLYLL VATYTEGMYP SICVEATESL GDYLRKKGS|
|Grade & Purity||> 95% pure|
|Molecular Weight||16.4 kDa (149aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Profilin-4, also known as PFN4, is a small actin-binding protein involved in the dynamic turnover and restructuring of the actin cytoskeleton. It is found in all eukaryotic organisms in most cells. Also, this is important for spatially and temporally controlled growth of actin microfilaments, which is an essential process in cellular locomotion and cell shape changes. Recombinant human Profilin-4, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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